As far as I am concerned, MS-DOS 6.22 is the latest
real stand alone version of DOS. Any number higher than that are stripped down versions bundled with Windows platforms.
Use DOSBox or use a real DOS platform.
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However the only versions of DOS currently recognized as stand-alone OSs, and supported as such by the Microsoft Corporation are DOS 6.0 and 6.22, both of which remain available for download via their MSDN, volume license, and OEM license partner websites, for customers with valid login credentials.
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Source.