64 bits Vista systems are retarded to the point they are unable to be able to run old 16 bits applications.
There is nothing you can do short of using a program to emulate the required state to use the game. DOSBox is such a program, which emulate DOS.
Do not hesitate to post here again would you require assistance with using DOSBox. If you are the least the patient type you could also simply read the readme inside the program and figure out everything by yourself. It is really not that complicated.
